Macroscopic observation , Seminal Reactive Oxygen Species NBT Staining Kit
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Macroscopic observation Rapid test for sperm superoxide anion (O2-.) Seminal Reactive Oxygen Species NBT Staining Kit
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The NBT test method is adopted in the form of chromogenic reactions, and the chromogenic product is derived from the formazan, the reductive substance of tetrazole.Semi-quantitative detection of ROS (O2− •) is based on the color of formazan.
Diagram of formazan deposit by NBT reaction of Sperm/leukocyte ROS (a) Negative sperm; (b) Sperm with positive head; (c) Sperm with positive midpiece; (d) Sperm with positive acrosome; (e) Weakly positive leukocyte; (f) Strongly positive leukocyte.
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