CE Vibrio Cholerae Rapid Test Kit O1/O139 Stool Detection Qualitative Rapid Test

The Vibrio cholerae Rapid Test is a rapid visual immunoassay for the qualitative, presumptive detection of Vibrio cholerae O1 and/or O139 in human fecal specimens. This kit is intended for use as an aid in the diagnosis of Vibrio cholerae O1 and/or O139 infection.

Principle

The Vibrio cholerae O1/O139 Antigen Rapid Test detects Vibrio cholerae O1/O139 through visual interpretation of color development on the internal strip. The test contain two strip in cassette, in each strip, anti- Vibrio cholerae O1/O139 antibodies are immobilized on the test region of the membrane. During testing, the specimen reacts with anti- Vibrio cholerae O1/O139 antibodies conjugated to colored particles and precoated onto the conjugate pad of the test. The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane. If there is sufficient Vibrio cholerae O1/O139 in the specimen, a colored band will form at the test region of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Operation

Bring tests, specimens, buffer and/or controlstoroomtemperature (15-30°C) before use.

1. Specimen collection and pre-treatment:

1) Best results will be obtained if the assay is performed within 6 hours after collection.

2) Unscrew and remove the dilution tube applicator stick.

3) Place the applicator stick back into the tube and screw the cap tightly. Be careful not to break the tip of the dilution tube.

4) Shake the specimen collection tube vigorously to mix the specimen and the extraction buffer.

2. Testing:

1) Remove the test from its sealed pouch, andplaceitona clean, level surface. Label the test withpatientorcontrol identification. To obtain a best result, theassayshould be performed within one hour.

2) Using a piece of tissue paper, break the tip of the dilution tube. Hold the tube vertically and dispense 2 drops of solution into the specimen well (S) of the test cassette.

Avoid trapping air bubbles in the specimen well(S), and do not drop any solution in observation window.

As the test begins to work, you will see color move across the membrane.

3) Wait for the colored band(s) to appear. The result should be read in 15 minutes.

3. Reading results:

Product Images

For further operation or performance details, please refer to final instruction manual.