Calprotectin Rapid Diagnostic Kit High-Accuracy Tumor Marker Rapid Test

Calprotectin Rapid Test Kit
 
 

 
【INTENDED USE】
The Calprotectin Rapid Test is a rapid chromatographic immunoassay for the qualitative detection of Calprotectin in human feces specimen which might be useful for the diagnosis of inflammatory gastrointestinal disorders.
 
【PRODUCTION INTRODUCTION】
Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8 and S100A9. The complex accounts for up to 60% of the soluble protein content of the neutrophil cytosol. Calprotectin becomes available in the intestinal lumen via leukocyte shedding, active secretion, cell disturbance, and cell death. This results in elevated faecal calprotectin levels, which can be detected in the stool. Elevated faecal calprotectin levels therefore indicate migration of neutrophils into the intestinal mucosa, which occurs during intestinal inflammation. Faecal calprotectin has been used to detect intestinal inflammation, and can serve as a marker for inflammatory bowel diseases. Calprotectin is useful as a marker, as it is resistant to enzymatic degradation, and can be easily measured in faeces.
 
【METHOD】
The Calprotectin Rapid Test Cassette (Feces) is a qualitative, lateral flow immunoassay for the detection of Calprotectin in human feces specimen. The membrane is precoated with anti-Calprotectin antibody on the test line region of the test. During testing, the specimen reacts with the particle coated with an Calprotectin antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-Calprotectin antibody on the membrane and generate a colored line. The presence of this colored line in the test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.
 
【OPERATION】
Bring tests, specimens, buffer and/or controls to room temperature (15-30°C) before use.
1. Specimen collection and pre-treatment:
  1) Best results will be obtained if the assay is performed within 6 hours after collection.
  2) Unscrew and remove the dilution tube applicator stick. Be careful not to spill or spattersolution from the tube. Collect specimens by inserting the applicator stick intoat least 4different sites of the feces to collect approximately 50 mg of feces (equivalent to 1/4of apea).
  3) Place the applicator stick back into the tube and screw the cap tightly. Be careful not tobreakthe tip of the dilution tube.
  4) Shake the specimen collection tube vigorously to mix the specimen and the extraction buffer.
2. Testing
  1) Remove the test from its sealed pouch, and place it on a clean, level surface. Label thetestwith patient or control identification. To obtain a best result, the assay should be performedwithin one hour.
  2) Using a piece of tissue paper, break the tip of the dilution tube. Hold the tube verticallyanddispense 2 drops of solution into the specimen well (S) of the test device.
Avoid trapping air bubbles in the specimen well (S), and do not drop any solutioninobservation window.
As the test begins to work, you will see color move across the membrane.
3. Wait for the colored band(s) to appear. The result should be read in 10 minutes. Do not interpretthe result after 20 minutes.
 
Note: If the specimen does not migrate (presence of particles), centrifuge the extractedspecimenscontained in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimenwell(S) of a new test cassette and start afresh following the instructions mentioned above.
 

4. Reading results:

【FEATURES】
 
1. Easy to use, no need extra medical equipment or trainee;
2. Quick detection, read in 10 minutes;
3. Affordable, highly cost-effective.

 
【MANUFACTURER】
Dewei Medical Equipment Co., Ltd
Adress: No.4 Building, Shiyou Industrial Park, Jun'an, Shunde, Foshan, China 528329
Email: sales@deweilab.com
 
For further operation or performance details, please refer to final instruction manual.