One Step Rotavirus Cassette Rapid Test Diagnostic By Feces Stool
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Onse Step Rotavirus Rapid Test Diagnostic Kit By Feces Stool
INTENDED USE The Rotavirus Rapid Test Device (Feces) is a rapid visual immunoassay for the qualitative presumptive detection of rotavirus in human fecal specimens. This kit is intended to be used as an aid in the diagnosis of rotavirus infection.
INTRODUCTION Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advancement in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oro-faecal route with an incubation period of 1-3 days. Although specimen collections taken within the second and fifth day of the illness are ideal for antigen detection, the rotavirus may still be found while diarrhoea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly, and immunocompromised patients. In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported. With hospitalised children suffering from acute entric disease up to 50% of the analysed specimen were positive for rotavirus. The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in diagnosing an infection. Instead, a variety of techniques have been developed to detect rotavirus in feces.
SAMPLE COLLECTION
TEST PROCEDURE Bring tests, specimens, buffer and/or controls to room temperature (15-30°C) before use.
For liquid specimens: Hold the pipette vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 50 µL) into the specimen collection tube containing the extraction buffer.
Avoid trapping air bubbles in the specimen well (S), and do not drop any solution in observation window. As the test begins to work, you will see color move across the membrane.
Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimens contained in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimen well (S) of a new test device and start afresh following the instructions mentioned above.
MAIN CONTENTS Rapid Test Cassette in Pouch Buffer Disposable dropper Package insert
STORAGE Store as packaged in the sealed pouch at room temperature or refrigerated (2-30°C). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date. FRQ: Rotavirus: 1) What are the first symptoms of rotavirus?
2) What causes rotavirus in adults?
3) How to get rid of rotavirus?
4) How long can rotavirus last?
6) Who is at risk for rotavirus?
7) What kills rotavirus?
8) How to detect rotavirus?
9) What food sources cause rotavirus?
10) Is milk good for rotavirus?
11) What color is rotavirus poop?
Adenovirus: 1) What is the cure for adenovirus?
2) What are the symptoms of adenovirus?
3) How long is contagious adenovirus?
4) Do you need antibiotics for adenovirus?
5) Is adenovirus harmful?
6) What organ does adenovirus affect?
7) How do you confirm adenovirus?
8) Does adenovirus cause cough?
9) What is the late phase of adenovirus?
10) Does adenovirus go away on its own?
11) Does adenovirus have a vaccine?
12) What is the best treatment for adenovirus?
13) What is the drug of choice for adenovirus? Ribavirin and cidofovir therapy have been used with variable success in immunosuppressed hosts. Anecdotal evidence suggests success against adenoviral infection with combined intravenous ribavirin and pooled human intravenous immunoglobulin. What are the benefits of adenovirus? 14) Adenoviruses are commonly used for gene transfer, as they can be generated at high titers and efficiently infect and express their genes in a variety of cell types including both dividing and quiescent cells.
15) What is the new treatment for adenovirus? For details, please contact Dewei staff for Instruction Manual!
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