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Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation

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Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation

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Brand Name SHIHONG
Model Number PEPTONE
Certification SGS
Place of Origin CHINA
Minimum Order Quantity 1MT
Price USD 2.3/KG - USD 3.1$/KG
Payment Terms L/C, D/A, D/P, T/T
Supply Ability 2,000MT/MONTH
Delivery Time 5 WORKING DAYS
Packaging Details 20KG/BAG 25KG/BAG
Detailed Product Description

Biological Grade Bone Peptone — High-Purity Nitrogen Substrate for Microbial Fermentation

Product Summary

Biological Grade Bone Peptone is a specialized nitrogen substrate derived from fresh bovine bone through a controlled enzymatic digestion process. This product serves as an essential nutrient base for industrial fermentation, microbiological culture media preparation, and biopharmaceutical applications, providing readily assimilable nitrogen sources in a highly soluble, low-residue format.

Production Methodology

 
 
Processing PhaseOperating Parameters
Raw Material Pre-treatmentFresh bovine bone selection, thermal sanitation, particulate reduction
Enzymatic DigestionPancreatic enzyme hydrolysis under controlled temperature conditions
Primary SeparationFractionation and initial clarification
Purification SequenceChromophore removal, odor reduction, membrane filtration
Concentration PathwayUltrafiltration, thermal concentration, sterile filtration
Final ProcessingAtomization drying, particle classification, quality verification

Functional Characteristics

The product exhibits the following functional attributes that support consistent microbial cultivation:

 
 
CharacteristicDescription
Nitrogen AvailabilityDual nitrogen fractions (total and amino) support both primary metabolism and specific biosynthetic pathways
Solution BehaviorComplete solubility with thermal stability; no precipitation under standard sterilization conditions
Mineral ProfileControlled sodium chloride and ash content minimize osmotic and inhibitory effects
Biological CompatibilitySupports growth of fastidious organisms without supplementation

Technical Specifications

 
 
ParameterUnitCertified LimitAnalytical Result
Total Nitrogen Fraction% (w/w)≥ 14.515.2
Amino Nitrogen Fraction% (w/w)≥ 2.53.1
Sodium Chloride Content% (w/w)≤ 5.02.56
Residual Moisture% (w/w)≤ 5.04.5
Mineral Residue (Ash)% (w/w)≤ 5.03.62
Hydrogen Ion Concentration (2% solution)pH units5.0 – 7.05.4
Visual Appearance—Yellow granular powderConforms
Aqueous Clarity (2%)—Clear solution, no sedimentConforms
Alkaline Stability (pH 8-9, 121°C)—No precipitationConforms

Strain-Based Performance Validation

 
 
Assay TypeTest MicroorganismExpected ReactionObserved Performance
Sulfhydryl Amino Acid DetectionSalmonella typhi / ShigellaPositive / NegativeMeets specifications
Tryptophan MetabolismE. coli / S. typhiPositive / NegativeMeets specifications
Glucose Metabolic Pathway (V-P)Enterobacter aerogenes / E. coliPositive / NegativeMeets specifications
Carbohydrate UtilizationE. coli / S. typhiNo acid formationMeets specifications
Erythrocyte InteractionS. pneumoniae / β-streptococcusAlpha / Beta patternsMaintained through passages
Chromophore ExpressionP. aeruginosa / S. aureusGreen / Gold pigmentationMaintained through passages
Colony DevelopmentE. coli / S. aureusDiameter ≥1.0mm, smooth morphologyMeets specifications
Minimum Inoculum ResponseStreptococcus / Gram-positive bacillusGrowth at 10⁻⁴ / 10⁻⁵ dilutionMeets specifications

Application Areas

 
 
SectorSpecific Applications
Industrial BiotechnologyMicrobial enzyme production, organic acid fermentation, single-cell protein cultivation
Diagnostic ManufacturingDehydrated culture media, selective media formulations, transport media
Pharmaceutical ProductionBacterial vaccine manufacturing, toxoid production, fermentor seed cultures
Research InstitutionsMicrobial physiology studies, strain development, media optimization

Physical Form and Storage

 
 
AttributeSpecification
Packaging Configuration20 kg units with moisture-protective inner liner and durable outer container
Storage ParametersTemperature-controlled environment (≤25°C), relative humidity ≤60%
Shelf Stability24 months from manufacture date under recommended conditions
Material HandlingMinimize atmospheric exposure; reseal containers promptly after use

Quality Framework

  • Internal Standard: Q/SCSH 001-2025

  • Reference Document: Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard (2000 Edition)

  • Analytical Methods: GB 5009 series (moisture, ash, nitrogen, pH, sodium chloride) and pharmacopoeial references

Values shown represent lot analysis. Performance characteristics may vary slightly between production batches. Technical consultation available for specialized application requirements.
Product Tags: High-Purity Bone Peptone   Biological Grade Peptone   Microbial Fermentation Nitrogen Substrate  
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