15×46mm Pediatric Blood Collection Tubes Ethylene Oxide Sterilization

Used for collection and storage of blood samples in biochemical, immune, serum, and other

clinical medical tests. Whether glass tube or plastic tube, its inner wall is extremely smooth,

can effectively avoid blood cells attached to the wall, minimize the specimen in vitro

hemolysis.

【SIZE AND USES】

Size:(3ml, 5ml, 7ml)

Plain, EDTA, Sodium fluoride, Heparin sodium, Sodium citrate, Fluoride Oxalate, ESR,

Thrombin, GEL SST.

【PACKING】

100 pieces per tray

【COLLECTION TUBES AND DESCRIPTION】

Whether you’re collecting your samples in-house or through a clinic, hospital, or pathology

center, you’ll need to have a good idea of what kind of blood collection tubes suit your purposes.

The first thing to check is your protocol—for example, some ELISAs will specify the types of

samples you can and can’t use.

But what if your protocol doesn’t specify, or you’re adapting a method from another system,

or you just want to make sure you’re storing the best type of sample for future not-yet-defined

analyses? Hopefully, I can help you start to find your way around all those differently colored

tubes.

A quick note about those cap colors before we begin: I’ve listed them below, and the

color-coding system is generally pretty consistent, but I can’t promise the colors are the

same in every company producing blood collection tubes, so always check first!

Serum Tubes

The first thing to figure out is whether you are after serum, or whether you’ll need to stop

the blood from clotting.

Don’t get serum confused with plasma—while they’re both the liquid, cell-free part of the

blood that can be obtained by centrifugation, the key difference is that serum is the product

of blood that has been allowed to clot, while in a plasma sample, the dense cells are simply

spun to the bottom.

Serum is, in simple terms, what remains in the blood after it clots: a cell-free liquid that is

also depleted of coagulation factors. It can be a good, stable way of measuring the blood’s

proteins, lipids, hormones, electrolytes, and so on. Many of these markers can be stored

for days in the fridge, or frozen down and measured in batches later.

• Serum (clot activator) tubes (color dependent on brand: BD is commonly gold but

          also red, Greiner is red). These tubes have silica particles, which activate clotting.

          Some also have a gel to separate the serum. Those without the separating gel are

          potentially more useful in sensitive diagnostic testing. If you’re looking for a

          protein that isn’t involved in coagulation, this is a good place to start.

• Thrombin-based clot activator tubes (orange). Although the silica-coated tubes 

          ​clot within about 30 minutes, the orange tubes clot within 5 minutes. They’re mainly

          used clinically for tests that are needed especially quickly. However, some of the serum

          components are a little less stable in these tubes.

Anticoagulant Tubes

This is the category to consider if you need cells or plasma (a cell-free liquid that still contains

coagulation factors).

EDTA (Purple)

EDTA prevents clotting by chelating calcium, an essential component of coagulation. This is

your basic hematology tube (by which I mean identifying and counting blood cells,

blood typing, etc).

Plasma stored from EDTA-treated blood can also be used to measure most proteins, and

genetic material can easily be stored from EDTA buffy coats (the interface between the red

cells and the plasma after centrifugation, containing white cells and platelets).

Note: these tubes contain either K2EDTA or K3EDTA.

Sodium Citrate (Light Blue)

For coagulation and platelet function tests. Like EDTA, citrate acts by removing calcium from

the blood. Unlike EDTA, it’s reversible—so calcium can be added back to study coagulation

under controlled conditions. Citrated plasma is also used to measure coagulation-relevant

factors.

It’s worth noting that a citrate tube should not be the first type of tube filled after

venepuncture—the first few mL of blood drawn will be a bit activated. If you only need

samples collected in citrate blood collection tubes for your project, then you should

collect a discard tube first.

Also, note that different concentrations of citrate are available from different companies.

CTAD (Also Light Blue)

CTAD stands for citrate, theophylline, adenosine, and dipyridamole. These aren’t very

commonly used but are worth knowing about—they prevent ex vivo activation of your

platelets, making them useful for some more sensitive platelet function and coagulation

studies. Note that CTAD is light-sensitive, so keep these guys in the dark.

Lithium/Sodium Heparin (Green)

Similar in use to serum clot activator tubes, but suitable for tests in plasma rather than

serum. Like the serum tubes, heparin tubes can also come with a separating gel. Heparin

acts by inhibiting thrombin formation. Note: if your endgame is PCR, you should know

that heparin is particularly known to interfere with PCR reactions.

However, whichever anticoagulant you choose, you may need to allow for it in your

reaction mix.

Sodium Fluoride (Color Dependent on Brand: BD is Grey, Greiner is Black)

Sodium fluoride is an antiglycolytic agent, so these tubes are used for glucose and lactate

testing. They also contain an anticoagulant (there are different types available).

Acid Citrate Dextrose—ACD (Yellow)

These ones are not common, but they are used for blood and tissue typing and DNA analysis.

Sodium Polyanethol Sulfonate—SPS (Also Yellow)

SPS stabilizes bacterial growth. Useful for microbiology.

For more specific purposes, there are more blood collection tubes out there, but,

hopefully, this has given you a handle on where to start. Good luck, and welcome to

the world of—let’s be honest—feeling just a little bit like a vampire.

Step of Correctly Preparing High-Quality Serum with Gel/Clot

Activator Tube

Step1:Place patient's arm in a downward position and clean venipuncture sire with alcohol；

Step2:Apply tourniquet；
Step3:Remove the upper cover of the needle and assemble the needle in the holder；

Perform venipuncture with arm downward and tube stopper upper-most；
Step4-5:Push tube onto the needle, puncturing stopper diaphragm；
Step 6； Release tourniquet as soon as blood appears in a tube and remove the tube from

the holder when the first tube has filed to its stated volume and blood flow ceases；
Step7:Turn the filed tube upside-down and return it to upright positions and repeat this

inversion according to different additive performance；
Step8:Place succeeding tubes in the holder, puncturing diaphragm to begin to follow, see

Recommended Order of Draw；
Step9:As soon as blood stops flowing in the last tube, remove the tube from the holder

and remove the needle from Vein, applying pressure to puncture site with the dry sterile

swab until bleeding stops；
Step10:Dispose the needles into Sharps Disposal Containers according to related policies

and guidelines.

【STORAGE CONDITIONS】

Store at temperature 5 - 25 ℃.

【INDEX OF SYMBOL】

【EXPORTER】

Magnus International Limited

F12, New City International Mansion A, 234 Huapao Ave.

Liuyang, Hunan Province 410300 China

Contact: Goodwellmedical@gmail.com

【AUTHORIZED REPRESENTATIVE】

Lotus NL B.V.

Koningin Julianaplein 10, 1e Verd, 2595AA, The Hague, Netherlands.