High Efficiency Magnetic Bead DNA Extraction Kits For PCR Applications

High Efficiency Magnetic Bead DNA Extraction Kits For PCR Applications

Fast and High Efficiency Magnetic Bead DNA Extraction Kit Purification Collection Kit for PCR Applications

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

Features:

Using silicon magnetic beads and buffer system with unique separation function, it can quickly, highly sensitively and efficiently extract high-purity nucleic acid from samples, and the extracted and purified nucleic acid can be used for PCR, RT-PCR, digestion, reverse transcription, and Southern Various common downstream experiments such as blot, applicable specimen types: whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid, etc. Applicable extraction instruments: most of the automatic nucleic acid extraction instruments on the market

benefit:

1. Magnetic bead-based technology isolates genomic DNA without the need for hazardous chemicals, centrifugation or vacuum manifolds, phenol and ethanol precipitation.

2. Fast and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes after sample preparation and lysis.

3. Simple cleavage with proteinase K without any mechanical cleavage.

4. Minimal RNA contamination.

5. Purified genomic DNA exhibits improved downstream performance in applications such as PCR.

6. A kit is included for automated processing of large numbers of samples in 96-well plates using a liquid handling robot.

Product Instructions:

1. Before using nucleic acid extraction reagents

①. Transfer proteinase k solvent to lyophilized powder containing proteinase k and mix well.
②Add 18ml and 42ml of absolute ethanol to CY3 and CY4 of CY-F006-10 (50preps-cells) and CY-F006-20 (50preps-saliva), stir well.
③. Add 36ml and 84ml of absolute ethanol to CY3 and CY4 of models CY-F006-11 (100preps-cells) and CY-F006-21 (100preps-saliva) and mix well.

2. Swab extraction steps:

① Dry swab collection, add 0.6ml CY1 solution and 10ul proteinase K, mix well, and incubate in a 65°C air incubator for 30 minutes (or wet collection: centrifuge the sample centrifuge tube containing the swab and preservation solution to 12000 Transfer for 1 minute, keep the precipitate, remove the supernatant, add 0.6ml of CY1 solution, 10ul of proteinase K, mix well, and incubate in an air incubator at 65 degrees Celsius for 30 minutes).
②. Remove the swab and centrifuge at 12000rpm for 1 minute.
③. Remove all supernatants into new centrifuge tubes and perform experiments.
④. Add 0.25ml of CY2 solution, 10ul of magnetic beads* (shake well before use), mix for 12min, put it on a magnetic stand for 30s, and blot dry.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6ml of CY4 solution, mix for 3min, put it on a magnetic stand for 30s, and dry the liquid
⑦, repeat steps ②⑥
⑧ Dry at room temperature for 10-20min, add 50ul CY5 liquid for elution, mix well, place it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube
⑨, measure the outer diameter

3. Saliva Extraction Step

① Centrifuge saliva plus preservation solution at 12000rpm for 1min
② Retain the precipitate and remove the supernatant
③. Add 0.6ml of CY1 solution and 10ul of proteinase k, stir evenly, and incubate in an air incubator at 65 degrees Celsius for 30 minutes.
④ Centrifuge at 12000rpm for 1min, take out all the supernatant into a new centrifuge tube, add 10ul of magnetic beads and 0.25ml of CY2, mix for 12min, and place it on a magnetic stand for 30s to absorb the liquid.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6 ml of CY4 solution, mix for 3 min, place it on a magnetic stand for 30 s, and absorb the liquid.
⑦, repeat step ⑥
⑧ Dry at room temperature for 10-20 minutes, add 50ul CY5 liquid for elution, mix well, put it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube.
⑨, measure the outer diameter

Note: To remove RNA, prepare RNaseA 10mg/ml: solvent (10mM sodium acetate: pH 5.0), boil for 15min, adjust pH 7.5 with Tris-HCl, and store at -20 degrees Celsius.

Precaution:

1. This product is only used for in vitro diagnosis.

2. The storage environment and extraction steps should be carried out in strict accordance with the instructions in the instruction manual.

3. If it is found that the amount is too small during the extraction process, the sample size can be appropriately increased or the number of extractions can be increased.

4. The extracted DNA must be fresh and tested in time.

Note: This transfer medium is intended for in vitro diagnostics and is not intended for internal or external use in humans or animals. If swallowed, it may cause a serious accident; it is irritating to eyes and skin. If splashed into eyes, rinse with water. It should be ventilated during use.