Human Ca125 Test Kit Elisa Carbohydrate Antigen 125

Test principle
Sandwich principle. Total duration of assay: 45 minutes. • Sample, Anti-CA12-5 coated microwells and enzyme labeled
Anti-CA12-5 are combined. • During the incubation, CA12-5 presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the CA12-5 molecules being sandwiched between
the solid phase and enzyme-linked antibodies. • After washing, a complex is generated between the solid phase,
the CA12-5 within the sample and enzyme-linked antibodies by
immunological reactions. • Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic
reaction is measured as absorbance. • The absorbance is proportional to the amount of CA12-5 in the
sample.